Growth factor composite scaffolds for bone defect repair via immediate implantation of bone defects

doi:10.3969/j.issn.2095-4344.2017.02.001

Abstract

Abstract:

BACKGROUND: Nerve growth factor has been shown to play an important role in bone healing, but little is reported on the effect of growth factor composite scaffolds via the immediate implantation in the repair of canine bone defects.
OBJECTIVE: To analyze the effect of nerve growth factor composite scaffolds via the immediate implantation for the repair of canine bone defects.
METHODS: Nerve growth factor composited strontium apatite scaffolds were prepared. Canine mandibular defect models were established and divided into three groups, followed by implanted with composite scaffold (experimental group), strontium apatite (positive control group), or nothing (blank control group). The three-dimensional CT reconstruction and hematoxylin-eosin staining of canine mandibular bone defects were observed.
RESULTS AND CONCLUSION: In the blank control group, there were few new bones surrounding bone defect. Trabecular bones spread from the defect center to the surrounding tissues in the experimental and positive control groups. The bone density, volume, thickness, and implant-bone contact were significantly increased, while the trabecular separation was significantly decreased in the experimental group compared with the positive control and blank control groups (P < 0.05), and all above indicators in the positive contro group were significantly higher than those in the blank control group (P < 0.05). Hematoxylin-eosin staining showed that in the experimental group, there were a large number of new bones that contacted with the surrounding bones closely, and trabecular bones arranged regularly. In the positive control group, newborn osteoid, trabeculare, and a small amount of debris were found. In the blank control group, few new bones were connected with the surrounding bones untightly and trabecular bone arranged irregularly. These results indicate that the nerve growth factor composite scaffold can promote the bone regeneration in the canine bone defects after immediate implantation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Nerve Growth Factor, Stents, Biocompatible Materials, Tissue Engineering

CLC Number:

R318

Yang Yu-peng1, Yang Sheng-jun2, Cheng Feng-xia1, Gu Jian-qi1, Zheng Yao1, Li Juan1, Hao Wei1, Wu Yong-sheng1 .

Growth factor composite scaffolds for bone defect repair via immediate implantation of bone defects

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 165-170.

Figures/Tables 4

References

[1]邸萍,林野,罗佳,等. 上颌前牙单牙种植修复中过渡义齿对软组织成型作用的临床研究[J]. 北京大学学报:医学版, 2012,44(1): 59-64.

[2]Happe A, Kunz A.Complex fixed implant-supported restoration in a site compromised by periodontitis: a case report.Int J Esthet Dent.2016;11(2):186-202.

[3]邱立新,胡秀莲,陈波,等.上颌窦底冲压提升法种植修复122例缺牙的临床观察[J]. 中华口腔医学杂志,2006,41(3):136-139.

[4]吴展,李婧,陈卓凡. 上颌前牙即刻种植即刻修复的临床应用研究[J]. 中国口腔种植学杂志,2012,17(2):67-71+82.

[5]Saito H, Chu SJ, Reynolds MA, et al.Provisional Restorations Used in Immediate Implant Placement Provide a Platform to Promote Peri-implant Soft Tissue Healing: A Pilot Study.Int J Periodontics Restorative Dent.2016;36(1):47-52.

[6]李伟. 美学区单牙即刻种植即刻修复与延期种植修复临床效果评价[D].安徽医科大学,2015.

[7]Levin BP, Wilk BL.The teamwork approach to esthetic tooth replacement with immediate implant placement andimmediate temporization.Compend Contin Educ Dent. 2015;36(9):682-688.

[8]Keady F, Murphy BP.Investigating the feasibility of using a grit blasting process to coat nitinol stents withhydroxyapatite.J Mater Sci Mater Med.2013;24(1):97-103.

[9]Bornapour M, Muja N,Shum-Tim D,et al.Biocompatibility and biodegradability of Mg-Sr alloys: the formation of Sr-substitutedhydroxyapatite.Acta Biomater.2013；9(2): 5319-5330.

[10]王冠聪. 具有二级三维网络结构的壳聚糖/羟基磷灰石骨组织工程复合支架材料的构建及其生物性能研究[D].山东大学,2012.

[11]Almeida JC, Wacha A, Gomes PS,et al.A biocompatible hybrid material with simultaneous calcium and strontium release capability forbone tissue repair.Mater Sci Eng C Mater Biol Appl.2016；1(62):429-438.

[12]Raucci MG, Alvarez-Perez M, Giugliano D,et al.Properties of carbon nanotube-dispersed Sr-hydroxyapatite injectable material for bone defects.Regen Biomater.2016；3(1):13-23.

[13]Sariibrahimoglu K, Yang W, Leeuwenburgh SC, et al. Development of porous polyurethane/strontium-substituted hydroxyapatite composites for boneregeneration.J Biomed Mater Res A.2015;103(6):1930-1939.

[14]Neves N, Campos BB, Almeida IF, et al.Strontium-rich injectable hybrid system for bone regeneration.Mater Sci Eng C Mater Biol Appl.2016;1(59):818-827.

[15]Meloni M, Cesselli D, Caporali A,et al.Cardiac Nerve Growth Factor Overexpression Induces Bone Marrow-derived Progenitor Cells Mobilization and Homing to the Infarcted Heart.Mol Ther.2015;23(12):1854-1866.

[16]Chen WH, Mao CQ, Zhuo LL, et al.Beta-nerve growth factor promotes neurogenesis and angiogenesis during the repair of bonedefects.Neural Regen Res.2015;10(7):1159-1165.

[17]King P, Maiorana C, Luthardt RG,et al.Clinical and Radiographic Evaluation of a Small-Diameter Dental Implant Used for the Restorationof Patients with Permanent Tooth Agenesis (Hypodontia) in the Maxillary Lateral Incisor and Mandibular Incisor Regions: A 36-Month Follow-Up.Int J Prosthodont.2016;29(2):147-153.

[18]胡秀莲,李健慧,邱立新,等. 先天缺牙患者种植修复[J]. 北京大学学报:医学版,2011,43(1):62-66.

[19]Stevens CJ.Technology to Control Excessive Occlusal Contact Force: Enhancing Implant RestorationLongevity.Dent Today.2016;35(1):112, 114, 116-117.

[20]徐欣.当代口腔种植修复技术新进展[J].口腔医学, 2015,35(4): 241-244.

[21]Khzam N, Arora H, Kim P, et al.Systematic Review of Soft Tissue Alterations and Esthetic Outcomes Following Immediate ImplantPlacement and Restoration of Single Implants in the Anterior Maxilla.J Periodontol.2015;86(12): 1321-1330.

[22]贺刚,陈治清,王培志,等. 改良式引导骨再生术+即刻修复技术在上前牙即刻种植中的应用评价[J]. 口腔医学, 2013,33(11): 739-742.

[23]苏媛,吕影涛,刘卫平,等. 美学区即刻种植即刻修复的临床应用[J].实用医学杂志,2014,30(11):1797-1799.

[24]许勇.可注射性纳米羟基磷灰石、壳聚糖复合材料与骨髓基质细胞生物相容性及其修复骨缺损的实验研究[D].南方医科大学, 2010.

[25]Alviar CL, Tellez A, Wang M,et al. Low-dose sirolimus-eluting hydroxyapatite coating on stents does not increase platelet activation and adhesion ex vivo.J Thromb Thrombolysis. 2012;34(1):91-98.

[26]Costa JR Jr, Abizaid A, Costa R,et al.1-year results of the hydroxyapatite polymer-free sirolimus-eluting stent for the treatment of single de novo coronary lesions: the VESTASYNC I trial.JACC Cardiovasc Interv.2009;2(5): 422-427.

[27]van der Giessen WJ, Sorop O, Serruys PW, et al.Lowering the dose of sirolimus, released from a nonpolymeric hydroxyapatite coated coronarystent, reduces signs of delayed healing.JACC Cardiovasc Interv.2009；2(4):284-290.

[28]Han J, Wan P, Ge Y, et al.Tailoring the degradation and biological response of a magnesium-strontium alloy for potentialbone substitute application. Mater Sci Eng C Mater Biol Appl.2016;1(58):799-811.

[29]Park JW, Kang DG, Hanawa T.New bone formation induced by surface strontium-modified ceramic bone graft substitute. Oral Dis.2016;22(1):53-61.

[30]Li Y,Shui X,Zhang L,et al.Cancellous bone healing around strontium-doped hydroxyapatite in osteoporotic rats previously treated with zoledronic acid.J Biomed Mater Res B Appl Biomater.2016;104(3):476-481.

[31]Boanini E, Torricelli P, Sima F,et al.Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses.J Colloid Interface Sci.2015;15(448):1-7.

[32]Monish B,Anthony L,Shilpa K.Immediate Implant Placement: Clinical Decisions, Advantages,and Disadvantages .J Prosthodontics.2008;17(7): 576-581.

[33]Faleh A,Stefan R,Noel C,et al.Effect of grafting materials on osseointegration of dental implants surrounded by circumferential bone defects. An experimental study in the dog.Clin Oral Implants Res.2008;19(4):329-344.

[34]Christophe DE,marie-Therese C,Christele C,et al.Surface enrichment of biomimetic apatites with biologically-active ions Mg2+ and Sr2+: A preamble to the activation of bone repair materials. Mater Sci and Eng C.2008;28(8):1544-1550.

[35]Gentleman E,Fredholm Y,Jell G,et al.The effects of strontiumsubstituted bioactive glasses on osteoblasts and osteoclasts in vitro.Biomaterials.2010;31(14):3949-3956.

[36]Goldenberg-Cohen N,Avraham-Lubin BC,Sadikov T,et al. Effect of coadministration of neuronal growth factors on neuroglial differentiation of bone marrow-derived stem cells in the ischemic retina.Invest Ophthalmol Vis Sci. 2014;55(1): 502-512.

[37]Wang L,Zhou S,Liu B,et al.Locally applied nerve growth factor enhances bone consolidation in a rabbit model of mandibular distraction osteogenesis.Orthop Res.2006;24(12): 2238-2245.

[38]王亮,曾剑玉,谭包生,等.神经生长因子在种植体周围早期骨愈合中的表达[J].北京口腔医学,2010,18(2):69-72.

[39]梅玲,汪琼,王恩群,等.神经生长因子对兔下颌骨缺损愈合影响的实验研究.口腔医学研究[J].2012,28(8)775-779.

[40]李俊杰,梅玲,王恩群,等.神经生长因子复合支架材料修复兔下颌骨缺损的实验研究. 现代口腔医学[J].2013,27(1)42-46.